Vol. 73 OXIDATION DURING DENATURATION OF OXYHAEMOGLOBIN
نویسنده
چکیده
light petroleum or carbon tetrachloride, but was extracted into butanol, in which the colour was stable for several days. The product of bufotenin oxidation remained in the aqueous phase with all the above-mentioned solvents including butanol. When a concentrated solution of serotonin was oxidized by concentrated denaturing oxyhaemoglobin, the filtrate was a turbid brownish pink which formed a black precipitate overnight. The addition ofascorbic acid to the pink product causes a rapid loss ofcolour, but the fluorescence does not return. The product was tested for possible biological activity on the heart of the clam Venu8 mercenaria, with essentially the bioassay procedure ofTwarog & Page (1953). No activity was found for the oxidized products of serotonin, bufotenin, 5-hydroxytryptophan or 5-HIAA.
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